Cell Signaling
Cleaved Caspase-3 (Asp175)
9661
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We are studying the cause of injury which occurs during cold ischemia when kidneys are stored in cold before transplantation. Histology shows the occurrence of apoptosis which we tried to reconfirm using caspase -3 blots.
Western Blot
Mouse kidney tissues
1:250 in 2% skimmed milk 4degree overnight
Skimmed milk 5%
1:1000 in 2% skimmed milk 2h at room temperature
5 min
ECL
Was not able to detect cleaved caspase-3 by western blots on mouse kidney samples. Though band was evident in positive control.
None
Was working with positive control
Was not able to pick up cleaved caspase-3 in mouse kidney samples
Can not be used for mouse kidney samples. Though the antibody says it reacts with mouse.