Cav1.2 Is Good Antibody

Anesthesiology
University of Wisconsin
Scientist

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Company:

EMD Millipore

Product Name:

Cav1.2 antibody

Catalog Number:

Ab5156

Aim of this research was to detect intensity of Cav1/2c protein in active zones of Substance P and Lectin positive neurons. This anti-Cav1/2 antibody was selected because company promised their high specificity. Result shows that Cav1/2 is not particularly increased in Substance P and Lectin (IB4) positive synapses.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse spinal cord

Primary Incubation

Overnight

Blocking Agent

Normal Donkey Serum 10%

Secondary Incubation

2 hours

Tertiary Incubation

None

Detection

Confocal microscopy

Results Summary

Cav1.2 is enriched in synapses of the dorsal horn.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Real staining

The Bad

None

The Bottom Line

Real detection of Cav1.2 channel

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