Cell Signaling Technology
Cyclin D1 (92G2) Rabbit mAb
2978
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Browse Similar Products: Cyclin D1 Western Blot Antibodies
Cyclin D1 is a marker of G0-G1 cell cylce arrest. We treated our cells with a known drug and observed its effect on cell cycle.
Western Blot
Breast cancer cell lysate, MCF7
1: 500 in blocking solution for overnight at 4 degrees
5% non fat dry milk in TBST
Anti rabbit HRP for an hour at room temperature
None
ECL
Cyclin D1 levels showed increase with time after treatment with drug thereby suggesting cell cycle arrest in MCF7 cells
Reproducible and no non specific background
It is a nice antibody and works pretty well.