Santa Cruz Biotechnology Inc.
anti-Mouse IgG-HRP
sc-2005
Browse Similar Products: IgG Western Blot Antibodies
This reagent is a medium sensitive secondary antibody. We analyze samples of crude Arabidopsis thaliana (mouse-ear cress) protein extract from seedlings or later developmental stages and bacterial extracts. We use this specific antibody to determine whether the target is expressed in the plant by standard western blotting after incubation with a suitable primary antibody. This antibody works ok for detection using decent dilutions of up to 1:500, we tested several comparable products from various companies.
Western Blot
Arabidopsis seedlings or leaves
1 to 1000, overnight
5% milk TBST
1 to 2500
n.a.
ECL
Proteins can be easily detected using this antibody, regardless whether recombinantly expressed in bacteria, or transiently or stably transformed and expressed in plants.
None
If optimized, good clear signal and reproducibility
Low concentration
Low cost secondary antibody