Staining PBMC with Anti-TCR Gamma/Delta FITC

August 23, 2016

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-human TCR gamma/delta FITC

Catalog Number:

331208

This antibody was used to phenotype T cell receptors in human PBMC. It was chosen because it is a commonly used clone, FITC is a bright fluorophore, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

5uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

TCR gamma/delta staining was as expected, with a very low percentage of T cells being positive in the FITC channel.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will continue to use this antibody.

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