R&D Systems
Anti-Myosin Heavy Chain
MAB4470
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Browse Similar Products: Myosin Heavy Chain Immunofluorescence Antibodies
This antibody was used in a study investigating the myopathic effects of stains using C2C12 myotubes. It has been published in Biochimica et Biophysica Acta in 2015 (Chu et al., Endothelial protective genes induced by statin are mimicked by ERK5 activation as triggered by a drug combination of FTI-277 and GGTI-298, Biochimica et Biophysica Acta. 1850, 1415-25). MAF4470 stained the 4% PFA fixed myotubes very nicely at a dilution of 1:50 for 2 hours followed by secondary staining using Alexa-488.
Immunofluorescence
Differentiated C2C12 cells
1:50, 1hr at room temperature in 2% donor goat serum
5% donor goat serum
Alexa-488, 1:400, 2hr at room temperature in 2% donor goat serum
N/A
Immunofluorescence (488nm)
Images of C2C12 stained with MAB4470 were capture and cell diameter was quantify using ImageJ. Statin-induced myopathy was analyzed as a reduction in cellular diameter.
None
Antibody gave great, clean signal
None to report
Antibodies worked great, would recommend.