Ebioscience
Anti-Human IFN gamma eFluor® 450
48-7319-41
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This antibody was used for phenotypic analysis of human PBMCs using minimal material. PBMCs were incubated with PMA/Iono/Brefeldin A for 4 hours and then stained with lymphocyte surface markers and the IFN gamma antibody. It served a good positive staining and I can highly recommend this antibody.
Flow Cytometry
PBMCs
After 4 hours stimulation cells were stained for 30 minutes with surface lymphocyte markers (CD3, CD4, CD8). then cells were permeabilized and fixed.
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After permeabilization cells were incubated for 30 minutes with the intracellular cytokine marker IFNgamma
Flow cytometry
This antibody worked well to detect PBMCs that produce IFN gamma.
The image shows CD4+ lymphocytes.
Reliable detection of IFNgamma producing lymphocytes
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This is a good antibody to use for intracellular cytokine staining of IFN gamma.