The Antibody Stains NK1.1+ Cells Very Well

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE anti-mouse NK-1.1 Antibody

Catalog Number:

108708

We wanted to study the immune cells in the intestine in a diseased vs healthy state, so we used this antibody to identify NK1.1+ cells by flow cytometry

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mesenteric lymph node cells

Primary Incubation

1:100

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody distinguishes NK1.1+ cells in the mLNs very clearly

Additional Notes

None

Related Categories

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Summary

The Good

The antibody stains NK1.1+ cells well

The Bad

None to mention

The Bottom Line

The antibody is good for identifying NK1.1+ NK cells

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