eBioscience
Rat IgG2a K Isotype Control FITC
11-4321
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I use flow cytometry to characterize immune cell populations in the lung and how they change following infection. I chose the antibody because I needed an isotype control and this product was recommended by eBioscience.
Flow Cytometry
Murine lungs
0.5 ug/10^6 cells, 20 minutes on ice
Anti-mouse CD16/32
No secondary
No tertiary
Flow cytometer
Seeing how this is a FITC antibody, I was expecting some background from dead cell or certain immune cells that can auto-fluoresce. I did not receive the cleanest results from this antibody and it is hard to separate all auto-fluorescence from background antibody binding. But overall I believe this antibody had minimal binding and I would use this product again.
None
Easy to use.
Hard to separate from auto-fluorescing cells.
This is still a good product and I would use it again.