Pan-Leukocyte Marker of Choice

July 01, 2016

Biological Sciences
University of Idaho
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor 488 anti-mouse CD45

Catalog Number:

103122

I am characterizing cell immune cell populations in the lung using flow cytometry. I selected this antibody because I wanted to make sure that I was only looking at immune cells and not total cells in the lung.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lungs

Primary Incubation

0.25 ug/10^6 cells, 20 minutes on ice

Blocking Agent

Anti-mouse CD16/32

Secondary Incubation

No secondary

Tertiary Incubation

No tertiary

Detection

Flow cytometer

Results Summary

I was able to determine what were immune cells from undesired cells in my samples. The CD45+ cells showed a clear separation on the flow cytometer and I was able to further gate them using other desired cell markers.

Additional Notes

None

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Summary

The Good

Easy to use, the recommended dilution works well but could be used at less.

The Bad

I liked this product, nothing really bad about it.

The Bottom Line

I will keep this antibody on hand for when I want to separate immune cells from non-immune cells. It makes a great distinction between the two.

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