Rabbit skin CD31 / PECAM-1 (Endothelial Cell Marker) IF staining

July 14, 2016

UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

ScyTek Laboratories Inc

Product Name:

CD31 / PECAM-1 (Endothelial Cell Marker); Clone C31.3 & JC/70A

Catalog Number:

RA0258-C.1

The aim is to identify blood vessels in rabbit skin using frozen skin section. The antibody is easy to use and the staining is specific.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rabbit ear skin

Primary Incubation

1/200 dilution, 45min, RT

Blocking Agent

10% goat serum

Secondary Incubation

Goat anti-mouse Alexa Fluor 594, 45min, RT

Tertiary Incubation

No

Detection

Fluorescence

Results Summary

Blood vessels in the skin were identified by this staining with little background.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy and specific

The Bad

None

The Bottom Line

Highly recommended for rabbit CD31 IF staining.

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