Works Good

Renal
University of Colorado
postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

XIAP antibody

Catalog Number:

2042

We have been working to find out how hibernators are protected from apoptosis. We investigated the role of inhibitor of apoptosis molecule XIAP to prevent apoptosis during hibernation. Since XIAP interacts caspase-3 directly we propose to use this.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Squirrel kidney cells

Primary Incubation

1:250 in 2% skimmed milk, 4 degrees overnight

Blocking Agent

Skimmed milk 5%

Secondary Incubation

1:1000 in 2% skimmed milk 2h at room temperature

Tertiary Incubation

5 min

Detection

ECL

Results Summary

Lane 1: squirrel kidney cells at 37 degrees Lane 2: squirrel kidney cells at 4 degrees

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Was able to detect XIAP in squirrel cell line

The Bad

The detection is not too great

The Bottom Line

It is a good product to detect the XIAP in squirrel cell line other than the usual species.

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