BioLegend Human IFN Gamma

June 28, 2016

Loyola University Chicago
Tumor Immunology
Grad student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Human IFN Gamma ELISA

Catalog Number:

430106

Our lab focuses uses TCR gene modified T cells to be used to adoptive T cell transfer. We retrovirally transduce activated T cells with an antigen specific TCR. We use these ELISA kits to measure functionality of the T cells.

Experimental Design and Results Summary

Application

Function of transduced T cells

Starting Material

N/A

Protocol Overview

ELISA kit was used to measure IFN gamma in supernatants of transduced T cells that had been co-cultured with target cells.

Tips

Double check concentrations of stock standard

Results Summary

We can measure the OD from the supernatants using the kits and determine pg/mL of IFN gamma.

Additional Notes

None

Image Gallery

Summary

The Good

The protocol could not be explained more simply

The Bad

The standard is very low, many dilutions of supernatant are needed

The Bottom Line

The protocol is extremely straight forward and spells out concentrations and how to dilute the reagents.

Join the discussion