CD11c FITC - Reliable Staining

Immunology
University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

CD11c FITC, clone N418

Catalog Number:

11-0114

We used this antibody to identify dendritic cell and macrophage subsets. Our research investigated antigen processing and presentation pathways in different murine cell types.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lymphocytes

Primary Incubation

1:500 for 20 minutes at 4 degC

Blocking Agent

2.4G2 Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Canto II flow cytometer

Results Summary

This antibody provides reliable, consistent staining on all colors tested. The attached picture shows CD11c vs CD11b staining on CD19neg lymphocytes.

Additional Notes

None

Image Gallery

Summary

The Good

Reliable, consistent staining

The Bad

None

The Bottom Line

Good option for CD11c staining

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