Good MHC II staining

Immunology
University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Mouse class II (I-A/I-E)

Catalog Number:

47-5321-82

We used this antibody to measure the level of MHC II on different populations of murine lymphocytes and splenocytes. Our research was investigating how antigens are processed and presented in different cell types.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lymphocytes

Primary Incubation

1:1000 dilution incubated for 20 minutes at 4 degC

Blocking Agent

2.4G2 Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Canto II flow cytometer

Results Summary

This antibody gives strong staining on the APC eF780 channel. It was specific staining.

Additional Notes

None

Related Categories

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Summary

The Good

This antibody gave strong staining on the APC eF780 channel

The Bad

None

The Bottom Line

Good staining, reliable antibody for a channel that does not always have good options available.

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