Great Antibody

June 21, 2016

Biological Sciences
University of Idaho
Graduate student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

Alexa Fluor 488 anti-mouse CD4

Catalog Number:

100423

I used this antibody in my flow cytometry panel in hopes of defining my CD4 T cell population. This antibody is a little nerve-racking because it recommends such a small amount to use, but it does give great results. My CD4 population was well separated and characterized because of this antibody. I would recommend this product and will continue to use it in the future.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lungs

Primary Incubation

0.06 ug/10^6 cells, 20 minutes on ice

Blocking Agent

Anti-mouse CD16/32

Secondary Incubation

No secondary

Tertiary Incubation

No tertiary

Detection

Flow cytometer

Results Summary

I was able to gate my CD3+CD4+ T cell populations in homogenized murine lungs. This could then be used for further analysis.

Additional Notes

None

Related Categories

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Summary

The Good

Very clear results and you can test many samples with a small volume.

The Bad

Very sensitive so it feels like you pipette nothing at times.

The Bottom Line

I would purchase this product again. To help with the small volumes, you can make a larger diluted stock and then it makes you feel a little more confident.

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