V450 Mouse IgG1, kappa Isotype Control Clone MOPC-21 Flow Cytometry Staining

June 24, 2016

Inflammation & Ageing
University of Birmingham
PhD Student

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Company:

BD Biosciences

Product Name:

V450 Mouse IgG1, κ Isotype Control Clone MOPC-21 (RUO)

Catalog Number:

560373

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This antibody was used to control for non-specific/Fc Receptor binding of human synovial cells analysed by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human synovial cells

Primary Incubation

30 minute incubation at 4oC. Live/dead stain.

Blocking Agent

No specific blocking step. 2% FCS in staining buffer.

Secondary Incubation

30 minute incubation at 4oC. Antibody cocktail used at this point containing antibody at 2ug/ml.

Tertiary Incubation

None

Detection

Mo Flow Astrios EQ 405 excitation 448/59 bandpass filter for emission

Results Summary

Antibody worked as expected for an isotype control with with no real non-specific/Fc receptor binding seen.

Additional Notes

None

Image Gallery

Summary

The Good

Antibody worked as expected.

The Bad

No particular bad points

The Bottom Line

A good isotype control

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