Time Course Effect of Chemoattractant on Par-4 Expression

June 15, 2016

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz

Product Name:

Par-4

Catalog Number:

sc-25466

Thrombin belongs to serine threonine family kinases. Thrombin exerts its effect via Pars and Par4 is one among them. Here we checked the time course effect of Par4 expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse cell lysate

Primary Incubation

1:1000 in 5% Milk prepared in 1x TBST

Blocking Agent

5% milk prepared in 1x TBST

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

NA

Detection

ECL

Results Summary

We found there is no increase in the expression level by chemoattractant.

Additional Notes

None

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Summary

The Good

This antibody is very good for western blot

The Bad

It gives lot of background and the band is not clear

The Bottom Line

This antibody is not good for western blot.

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