Time Course Effect of Chemoattractant on Cul-3 Expression

June 13, 2016

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz

Product Name:

Cul-3

Catalog Number:

sc-166054

Cullins are the largest family of Ubiquitin E3 ligases which has different cellular functions. Here in this study we checked the time course effect of Cullins by chemoattractant.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

Mouse cell lysate

Primary Incubation

1:1000 in 5% Milk prepared in 1x TBST

Blocking Agent

5% milk prepared in 1x TBST

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

N/A

Detection

ECL

Results Summary

We found time dependent increase in the cullin3 expresssion.

Additional Notes

None

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Summary

The Good

This antibody is very good for immunoprecipitation and western blot.

The Bad

We had to use at least 1:500 concentration to detect the band prominently.

The Bottom Line

This antibody is perfect for western blot and also immunoprecipitation, it works great. Highly recommended.

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