Cell Proliferation Protein Marker

Department of Urology
UT Southwestern Medical Center
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Ki-67 AF700

Catalog Number:

561277

Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD325+ cells. Leukocytes were looked at using CD326-CD45+. Sub-populations (such as epithelial stem cells or individual leukocyte populations like T-Cells) were gated with appropriate markers.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary human prostate cells

Primary Incubation

Recommended for 20-30mins

Blocking Agent

FcX

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometer

Results Summary

Ki67=Proliferating cellshu prostate not very proliferative, but saw no positive cells

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

No Positive cells

The Bottom Line

Did not work for my tissue

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