EMD Millipore
SNAP i.d. 2.0 Protein Detection System
SNAP2MINI
Info: View Product View Product Specs
We are constantly performing western blots in my lab trying to find new biomarkers for molecular imaging purposes. We were told that this instrument can drastically reduce the time needed for blocking, primary antibody incubation, and secondary antibody incubations.
Western blot
Nitrocellulose membrane with protein, blocking agent (LiCor PBS Blocking Solution), Membrane Inserts
After you have transferred the protein to nitrocellulose membrane, place the membrane into the membrane holder. Place the membrane holder into the instrument chamber. Add blocking solution, turn on vacuum. Next, place 5 mL of primary antibody incubation for 10 min, turn on vacuum. Next, wash four-times with 30 mL wash buffer. Next, add 5 mL secondary antibody solution and allow to incubate for 10 min, turn on vacuum. Wash four-times before scanning.
I don't have any tips because we can still not get it to work.
We have not been able to get this instrument to work. While the vacuum works correctly, the system is not even so the antibody does not get to the entire membrane. In addition, we have tried using this system over 10-times; yet we have only had 1 western blot that slightly worked. We believe the traditional method is much better than this system.
This system does not contain any great features. We were hoping that it would save time.
None
System is cheap
System does not work and shipping takes over a month.
Don't get this system....keep with the traditional methods or look into Life Technologies iBind system (we previously wrote a review on this system -- 4 stars).
Product Name: SNAP i.d. 2.0 Protein Detection SystemSupplier Page