Brilliant Violet 785™ Anti-Human CD14 Antibody Flow Cytometry Staining

Inflammation & Ageing
University of Birmingham
PhD Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 785™ anti-human CD14 Antibody

Catalog Number:

301840

CD14-BV785 was used to investigate human synovial cells yielded by digestion

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human synovial cells

Primary Incubation

30 minute incubation at 4oC. Live/dead stain.

Blocking Agent

No specific blocking step. 2% FCS in staining buffer.

Secondary Incubation

30 minute incubation at 4oC. Antibody cocktail used at this point containing antibody at 5ug/ml.

Tertiary Incubation

None

Detection

BD LSR X-20 Fortessa. Analysis using FlowJo v10

Results Summary

Staining worked very well producing a bright population along with lower expressing cells. Even though the cells yielded by digestion can be difficult to work with, the antibody performed well.

Additional Notes

None

Related Categories

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Summary

The Good

Antibody worked well on difficult cells and produced very bright population

The Bad

No particular negative

The Bottom Line

Very well performing antibody

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