Cell Signaling Technology
Phospho-YAP (Ser127) Antibody
3008
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Mouse pups were subjected to low oxygen concentration for different time periods. Pups kept at normal room air were taken as control. At specific time periods eyes were enucleated, retinal extract were made, and equal amounts of proteins were resolved in 10%SDS-PAGE followed by immunoblotting and detection phospho-YAP (Ser127) levels using its specific antibody obtained from Cell Signaling Technology
Western Blot
Mouse whole tissue lysate
Dilution (1:1000) in 5% BAS prepared in TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in TBST
Dilution (1:4000) in 5% skimmed milk prepared in TBST, 1hr at RT
NA
ECL detection in X-Ray film
In the present study, antibody from cell Signaling technology was used for immudetection which showed excellent specificity towards phospho-YAP (Ser127) in mouse tissue. Although, a significant response of treatment on phospho-YAP (Ser127) levels were observed.
None
Detected highly specific band of phospho-YAP (Ser127)
Got somewhat diffused band
Excellent antibody for detection