CD11c_APC-Fire750

Department of Pediatrics
University of California, San Diego
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC/Fire™ 750 anti-mouse CD11c Antibody

Catalog Number:

117352

Browse Similar Products: CD11c Flow Cytometry Antibodies

This is a great replacement for APC-Cy7 or APC-H7 for flow cytometry, it works well and as advertised.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lung or lymph node

Primary Incubation

1:200 at 4C for 20 minutes

Blocking Agent

FcBlock in FACS buffer

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry: APC-Cy7 Channel

Results Summary

CD11c staining worked as normal. APC-Fire750 had less compensation issues with APC than other conjugates I've used in the past. I also used this with fixation (4% PFA), Click-IT reaction (which has copper and saponin perm), and intranuclear staining (FoxP3 Perm buffer) - none of them seemed to affect the staining brightness or compensation. Very happy with the Ab / conjugate

Additional Notes

Works as advertised

Related Categories

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Summary

The Good

Easy to use, no troubleshooting needed

The Bad

None

The Bottom Line

Would use again

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