Cell Signaling Technology
p-mTOR 2481
2974
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Browse Similar Products: Phospho-mTOR Western Blot Antibodies
This is used for detecting endogenous levels of mTOR only when phosphorylated at serine 2481. 1:500 dilution could be the primary choice to try on it.
Western Blot
Tissue lysis or cell lines lysis
1:500 dilution, incubation overnight at 4 degrees
5% BSA
Goat anti rabbit antibody, 1:10000 dilution for incubation 1h at room temperature
NA
ECL
Followed the protocol, the band is not strong, so the exposure time usually needs longer time, the background could be adjusted through changing the secondary antibody dilution ratio, washing buffer washing times and various ECL subtract reagents.
None
A good antibody
Since it's a lare sized protein, it's not easy to get a clear or beautiful band each time. We should be careful on protein lysis preparation, and try different developing reagents.