Cell Signaling Technology
P-EIF4E
9741S
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Antibody Phospho-eIF4E (Ser209) is used for detecting endogenous levels of eIF4E only when phosphorylated at serine 209. eIF4E binds to the mRNA cap structure to mediate the initiation of translation.
Western Blot
Tissue lysis or cell lines lysis
1:1000 dilution, incubation overnight at 4 degrees
5% BSA
1:10000 dilution of anti-rabbit secondary antibody, 1hour at room temperature
NA
ECL
There is an approximately 25kda size band when developed with ECL, and it is usually over-expressed in tumor tissues when akt /mTOR pathway were activated.
None
A good antibody.
If 1:1000 dilution does not work well, the dilution of primary antibody could be reduced to 1:500.