A Reliable Antibody for Immunohistochemistry Staining on GS

May 18, 2016

pathology
upmc
research associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

GS

Catalog Number:

sc-74430

This is a poly-antibody from Santa Cruz Biotechnology; the dilution for IHC is about 1:50 to 1:100 in accordance with the IHC protocol.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Paraffin block slides

Primary Incubation

1:1000 dilution, incubation overnight at 4 degrees

Blocking Agent

5% BSA or 5% non-fat milk

Secondary Incubation

Goat anti-mouse antibody (1:10000 dilution)

Tertiary Incubation

NA

Detection

ECL

Results Summary

The dilution can be changed based on the background.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

This is a good antibody.

The Bad

None

The Bottom Line

None

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