Best Bicinchoninic Acid (BCA) Protein Quantification

May 17, 2016

UFPR
Patologia Básica
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Company:

G Bioscience

Product Name:

Bicinchoninic Acid (BCA) Protein Assay

Catalog Number:

786-571

Determination of protein concentration is essential for molecular assays in bioscience. The BCA assay from G bioscience is very easy to standardize, both in tubes and in microplates.

Experimental Design and Results Summary

Application

Quantitative protein determination

Starting Material

Jejunum of chickens

Protocol Overview

The jejunum of chickens are fragmented and resuspended in lysis Buffer 1X. Next, we use the Bicinchoninic Acid (BCA) Protein Assay from G Bioscience to quantify total proteins.

Tips

Keep the samples on ice all the time. If possible, use protease inibitors in the lysis buffer.

Results Summary

The standard curve is very well (r²=0,998). And the triplicate of samples has a very low Standart desviation indice.

Additional Notes

None

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Summary

The Good

It is very high sensitive and very easy to use.

The Bad

Sometimes it is hard to find a supplier and some detergents influence the results

The Bottom Line

This kit is very easy to use and even a student that is starting can do it. Also, it is very sensible.

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