DNA Extraction from Soil Samples

May 17, 2016

Durban University of Technology
IWWT
Post doctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

MO BIO Laboratories

Product Name:

PowerSoil ® DNA Isolation Kit

Catalog Number:

12888-50

I am working on the development of molecular techniques for the detection of bacteria in the environmental samples. The PowerSoil ® DNA Isolation kit was used in extraction of total DNA from complex environmental samples like soil.

Experimental Design and Results Summary

Application

DNA extraction

Starting Material

None

Protocol Overview

Protocol mentioned is simple and easy

Tips

Homogenization of soil samples prior to DNA extraction will help in getting better results

Results Summary

The results obtained were good in terms of quality of DNA.

Additional Notes

None

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Summary

The Good

Easy protocol and kit effectively removes PCR inhibitors during the extraction procedures

The Bad

Some time overall yield of DNA was found to be low

The Bottom Line

This kits effectively removes PCR inhibitors during the DNA extraction procedures and resulted in good quality DNA

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