STEMCELL
EasySep™ Human CD4+ T Cell Enrichment Kit
19052
Info: View Product View Product Specs
While there are a number of methods to separate/sort out lymphocytes from peripheral blood cells, isolating CD4+ specifically with a high degree of purity may prove difficult. The kit by STEMCELL allows for an easy isolation of CD4+ t-cells for the utilization of different assays (e.g. regulatory T-cell assays/induction). While the kit may be expensive, it may be fruitful in situations where a FACS machine is not available.
CD4+ cell sorting
Peripheral Blood Mononuclear Cells
1) Incubate peripheral blood mononuclear cells with 50uL of enrichment media for 10 minutes2) Incubate the concoction with 100uL of magnetic beads for 5 minutes3) Place in magnet for 5 minutes and pour out the unbound CD4+ lymphocytes
If a high cellular density is required, resuspend the beads in fresh media after pouring out the selected lymphocytes and re-incubate in the magnet for 5 minutes for a 2nd pour
Isolated pure CD4+ lymphocytes with high viability (>95%)
Easy to use, high viability, pure CD4+ lymphocyte population
Expensive
Perfect sorting tool for regulatory T-cell assays or for other in vitro assays where a pure CD4+ lymphocytic population is preferred.
Product Name: EasySep™ Human CD4+ T Cell Enrichment KitSupplier Page