Best Anti-Fg Serum

Molecular Micro
Wash U
Postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

Anti-Fibrinogen antibody produced in goat

Catalog Number:

F8512

Browse Similar Products: Anti-Fibrinogen Western Blot Antibodies

I tried multiple anti-fibrinogen antibodies in a row, and this is the best one for my experiments. It is a whole antiserum from goat, I was expecting it to have background, but it has actually the least background produced. I have a tough sample to study, this one fits my critique. Although the dilution factor is relative high, I use 1:500 to 1:1000, but it is still a great price.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Homogenized tissue

Primary Incubation

1:500-1:1000

Blocking Agent

5% milk

Secondary Incubation

1:2000

Tertiary Incubation

NA

Detection

ECL

Results Summary

As for a homogenized animal tissue, there are tons of other proteins existing. This antibody gives the most clean background, without diminishing detection signal.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Relatively clean background

The Bad

High dilution factor

The Bottom Line

Works great!

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