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Alexa Fluor® 700 anti-mouse CD45 Antibody
103127
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This antibody was used to identify hematopoietic cells in murine lung. Obtained very consistent results.
Flow Cytometry
Murine lung, bone marrow, lymph node
1:200 at 4C for 20 minutes
FcBlock in PBS or FACS buffer
NA
Flow cytometry
Very consistent results. I often leave this Ab out of my staining mix - I don't see any difference in my end results if I include it or not (i.e. I'm looking at immune cells in lungs, and the other markers I gate on are distinctive enough that all the cells I'm ultimately gating are CD45+, so this isn't really needed). But, it is nice to have and it works well in Alexa700 - which not all Antigens can be reliably detected in. Overall, very happy with the Ab, no complaints. Image shown is an infected lung (murine)
None
Reliable antibody
Happy with the product