Bosterbio
Anti-Tryptophan Hydroxylase antibody (monoclonal)
MA1099
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The goal was to identify the dorsal raphe in rat brains, an area rich in serotonergic neurons, so that I could determine double-label in that region. I had been using an AB from Abcam, but they stopped making it. Found this product with a google search, and the vendor had a fair return policy.
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Immunohistochemistry
Wistar rat brain
1:50, overnight at room temperature.
5% normal goat serum
1:500 Alexa Fluor 594
NA
Fluorescence
Strong signal that identifies dorsal raphe; aqueductus alone is not sufficient landmark.
None
Outstanding signal of cell bodies and processes
Unsure of application in paraffin
Great for free floating sections fixed in 4% paraformaldehyde. Probably can be used at less than 1:500.