Acceptable Level of Single Cell Isolation from Lung

The Jackson Laboratory
Cancer biology
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotech

Product Name:

Lung Dissociation Kit

Catalog Number:

130-095-927

The lung Dissociation kit yielded a decent number of single cells from the mouse lung following the protocol that comes with this kit. We used this kit to study isolated single cells from the mouse lung for a transcriptome study.

Experimental Design and Results Summary

Application

Isolation of epithelial single cells

Starting Material

Mouse lung

Protocol Overview

The manual that comes with this kit is optimized for mouse lung tissue. Tissue preparation is critical (perfusion) for obtaining highly viable cells. It needs MACS dissociator as an additional instrument.

Tips

Take indicated amount of tissue

Results Summary

We obtained single epithelial cells for the genomics study.

Additional Notes

None

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Summary

The Good

Decent yield.

The Bad

None

The Bottom Line

Recommended for epithelial cell isolation from mouse lung tissue.

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