Review of Anti-GRK6 Antibody (cat#PB9709)

April 06, 2016

Emergency Medicine
Thomas Jefferson University
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Bosterbio

Product Name:

Anti-GRK6 Picoband™ Antibody

Catalog Number:

#PB9709

Through rat endothelial cells' western blot tests, we can see PB9709 Anti-GRK6 antibody had a high background so that the blot can hardly be seen.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Rat endothelial cells

Primary Incubation

Anti GRK6 (PB 9709) at 1 ug/ml,4˚C overnight

Blocking Agent

5%(w/v) milk in TBST

Secondary Incubation

Anti-rabbit IgG(cell signaling,#7074) at 1:1000, 2h, room temp

Tertiary Incubation

NA

Detection

Kodak Image Station 4000R Pro;Thermo Scientific West Femto Maximum Sensitivity Substrate(#34096)

Results Summary

The target band is hard to be detected.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

There is none, so far.

The Bad

The target band can hardly be detected.

The Bottom Line

With the results of the western blots, we don't recommend using it to detect rEC's Grk6 protein .

Share your experience with other scientists. Write a Review! »

Join the discussion