Good Antibody for I-A/I-E Staining

March 31, 2016

Internal Medicine
University of California
Post Doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 anti-mouse I-A/I-E Antibody

Catalog Number:

107621

Mouse splenocytes injected with DNA were checked for the presence of a specific class of MHC.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:300 in Fc block for 2 hours

Blocking Agent

CD16/32

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

AF-700

Results Summary

There is less leakage in any channel and detection is good with this color fluorochrome

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use, good color

The Bad

None

The Bottom Line

Cheap and good color for fast detection with flow cytometry.

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