Easily Distinguishes Murine CD3e+ Peripheral Blood T Cells

Immunology
Duke University
Predoctoral

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse CD3e PE

Catalog Number:

12-0031-81

The goal of this experiment was to clearly distinguish T cells in the peripheral blood of female C57Bl/6 mice for subsequent phenotyping. I selected this product because CD3e is a T cell-specific surface marker.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Peripheral blood leukocytes

Primary Incubation

Stained cells in 100 uL cold PBS containing Live/Dead and TruStain Fc block (2 ug/mL) on ice for 15 minutes.

Blocking Agent

TruStain Fc block (2 ug/mL)

Secondary Incubation

Added 100 uL cold PBS containing isotype control (4 ug/mL) or CD3e PE (4 ug/mL) and incubated on ice for 15 minutes

Tertiary Incubation

NA

Detection

Flow Cytometer

Results Summary

CD3e+ peripheral blood leukocytes were fairly easy to distinguish from CD3e- peripheral blood leukocytes, though there was not complete separation between the two populations.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

It's very inexpensive and sufficiently separates CD3e+ cells for gross enumeration.

The Bad

It does not completely separate CD3e+ cells from CD3e- cells.

The Bottom Line

If you are simply enumerating cell populations based on surface marker expression (e.g., T cells, B cells, macrophages, etc.), this antibody is great. If you need to be very confident that you are only looking at T cells, you should probably select a different antibody.

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