Amazing Antibody for CD31-Staining of Tumor Tissue

March 25, 2016

Medical Physics
University of Wisconsin
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 488 anti-mouse CD31

Catalog Number:

102514

Our lab focuses on the molecular imaging of cancer with antibodies and nanoparticles. To show that the nanoparticle-based imaging agent is reaching the tumor, we must stain the tissues to determine the spatial location. In addition, we stain the vasculature to determine the imaging agent was able to effectively extravasate. We chose this product because the company provides great customer service.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Tumor tissue from U87mg mouse model

Primary Incubation

Alexa Fluor® 488 anti-mouse CD31 (5 µg/mL) for 1 h at 25 °C

Blocking Agent

10% donkey serum for 30 min at 20-25 ºC

Secondary Incubation

DAPI-containing mounting medium was applied and the slides were cover-slipped

Tertiary Incubation

There was no tertiary incubation.

Detection

Nikon A1R + Confocal Microscope and imaging analyzed using the NIS-Elements Ar with Deconvolution software package

Results Summary

CD31 and DAPI were visualized in the imaging with DAPI represented as blue and CD31 in green.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Excellent company with great customer service, simple to use.

The Bad

Not the best CD31 antibody for staining as it is slightly grainy.

The Bottom Line

We recommend this antibody as an option for CD31 staining of tissue sections. While it is not our first choice, it is in our top 5 list.

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