Foxp3+ T Cells in the Spleen are Clearly Stained with the Antibody

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse/Rat Foxp3 PE

Catalog Number:

12-5773-82

We wanted to determine the proportion of Treg cells in the spleen in a healthy versus a diseased state. To do this, we wanted to examine the proportion of Tregs using flow cytometry, requiring an antibody capable of staining Foxp3+ T-cells well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of splenic cells

Primary Incubation

1:100 in staining buffer for 25 minutes at 4 degrees

Blocking Agent

FcR Block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD LSR II flow cytometer

Results Summary

The antibody distinguished the population of Foxp3+ T-cells very well.

Additional Notes

The cells shown are pre-gated for CD3 positivity. Cells were stained using the eBioscience Foxp3 Staining Kit.

Related Categories

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Summary

The Good

Stains Foxp3+ T-cells well.

The Bad

None to mention

The Bottom Line

Good antibody for identifying Tregs/Foxp3+ T-cells

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