Eppendorf Has a Great ThermoMixer for All Applications

March 24, 2016

University of Wisconsin
Medical Physics
Postdoctoral Fellow

Overall

Performance

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Eppendorf

Product Name:

ThermoMixer F1.5, with thermoblock for 24 reaction vessels (1.5mL)

Catalog Number:

5384000020

The focus of our lab is molecular imaging of cancer with antibody-based imaging agents. To do this, we must digest antibodies to obtain F(ab')2 or Fab. This enzymatic reaction requires a temperature of 37 degrees Celcius with constant agitation. In addition, radiolabeling of antibodies must be done with the same conditions. Currently, we have two of these systems in our lab. We chose this system because Eppendorf has a great reputation for providing elite products that last a long time.

Experimental Design and Results Summary

Application

Antibody digestion and radiolabeling of antibodies

Starting Material

Full antibodies for digestion and chelated antibody + radioisotope for radiolabeling

Protocol Overview

We use the Fab or F(ab')2 kit for antibody digestion (ThermoFisher Scientific). After addition of the enzyme, we incubate the samples for 5 hour at 37 degrees Celsius on the Eppendorf ThermoMixer F1.5 at 550 rpm. For antibody radiolabeling, we add the chelated antibody to a 1.5 mL tube with the radioisotope. After changing the pH (isotope-dependent), we place the tube on the Eppendorf ThermoMixer F1.5 for 1 hour at 37 degrees Celsius and 550 rpm.

Tips

The machine is simple to use and it has buttons at the top with pre-set temperatures.

Results Summary

We have successfully digested many enzymes. To confirm the digestion, we run the protein on a gel. In addition, we have never had any problems with radiolabeling. We have never had any problems with the ThermoMixer changing temperatures or speeds. Our last ThermoMixer lasted 9 years and we expect our two new ThermoMixers to last the next decade.

Features Summary

The system is simple to use and provides short cut buttons at the top for different temperatures.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Excellent manufacturing, reasonable price, consistent temperature, and speed

The Bad

There are no bad things to mention about this system.

The Bottom Line

I highly recommend the Eppendorf ThermoMixer for use in labs. We have never had any problems with our system.

Join the discussion