Time Course Effect of Chemoattractant on GSK-3ß Phosphorylation at Position Ser9

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Cell Signaling Technology

Product Name:

Phospho-GSK-3β (Ser9) Antibody

Catalog Number:

9366S

Glycogen Synthase kinase (GSK-3β) is a ubiquitously expressed serine/threonine kinase that phosphorylates and inactivates glycogen synthase. GSK-3β activity is inhibited by phosphorylation of an amino-terminal serine residue. Here we checked the time course effect of GSK-3β on serine phosphorylation by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse cell lysate

Primary Incubation

1:1000 in 5%BSA prepared in 1xTBST

Blocking Agent

5% BSA prepared in 1xTBST

Secondary Incubation

1:4000 5%Milk prepared in 1XTBST

Tertiary Incubation

NA

Detection

ECL

Results Summary

We found time dependent increase of serine phosphorylastion in GSK-3β by chemoattractant.

Additional Notes

None

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Summary

The Good

The antibody works effeciently and the results are accurate and easy to optmize.

The Bad

None

The Bottom Line

This antibody is efficient for performing western blots and the signal is great. Highly recommended.

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