Time Course Effect of Chemoattractant on GSK-3ß Tyrosine Phosphorylation

March 23, 2016

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

Anti-GSK-3β (pY216)

Catalog Number:

612312

Glycogen Synthase Kinase-3β (GSK3β) is a serine/threonine kinase which affects glycogen metabolism by phosphorylating various signaling events by downregulating muscle glycogen syhthase. Regulation of tyrosine phosphorylation is important for cell death or survival. Here we checked the time course effect of GSK-3β on tyrosine phosphorylation in mouse cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse cell lysate

Primary Incubation

1:1000 in 5%BSA prepared in 1xTBST

Blocking Agent

5% BSA prepared in 1xTBST

Secondary Incubation

1:4000 5%Milk prepared in 1XTBST

Tertiary Incubation

NA

Detection

ECL

Results Summary

We found that there is no significant increase in the tyrosine phosphorylation of GSK-3β by chemoattractant.

Additional Notes

None

Related Categories

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Summary

The Good

The antibody works effeciently and the results are accurate and easy to optmize.

The Bad

None

The Bottom Line

GSK-3β tyrosine phosphorylation is important for cell death and proliferation. This antibody works great in detecting the tyrosine phosphorylation of GSK-3β. Highly recommended.

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