Time Course Effect of Chemoattractant Induced Ubiquitination in Mouse Cells

March 21, 2016

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Ubiquitin Antibody

Catalog Number:

3933S

Ubiquitin is a highly conserved polypeptide and plays an important role in the proteosome pathway. Here we checked the time course effect of ubiquitin by chemoattractant in mouse cells.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

Mouse cell lysate

Primary Incubation

1:1000 in 5%Milk prepared in 1xTBST

Blocking Agent

5% Milk prepared in 1x TBST

Secondary Incubation

1:4000 5%Milk prepared in 1XTBST

Tertiary Incubation

NA

Detection

ECL

Results Summary

We found that time dependent degradation by chemoattractant in mouse cells.

Additional Notes

None

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Summary

The Good

The antibody works effeciently and the results are accurate and easy to optmize.

The Bad

None

The Bottom Line

This Ubiquitin antibody is helpful in using immunoprecipitation experiments to know whether that particular molecule of interest is involving in ubiqutination or not.

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