Anti-Mouse CD11c Antibody

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse CD11c Antibody

Catalog Number:

117310

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Lymphocytes

Primary Incubation

1:50 dilution for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

Observed CD11c expression on lung lymphocytes in response to mycobacterial antigens and helped in studying Dendritic cell population.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Good flow cytometry antibody

The Bad

None

The Bottom Line

Efficient CD11c expression detection for studying DCs

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