APC Anti-Mouse CD8a Antibody

March 15, 2016

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

APC anti-mouse CD8a Antibody

Catalog Number:

100712

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Lymphocytes

Primary Incubation

1:100 dilution for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

Used to detect CD8+ sub-populations of murine lymphocytes.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

No cross reactivity, gives nice signal

The Bad

None

The Bottom Line

Good antibody for surface staining of CD8

Share your experience with other scientists. Write a Review! »

Join the discussion