No Staining with CD27 Antibody

Immunology
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV605, anti-human CD27

Catalog Number:

562656

CD27 surface and intracellular expression was assessed on human monocyte-derived dendritic cells. This product was selected because it was available with this fluorochrome.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Monocyte derived dendritic cells

Primary Incubation

30 min on ice

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

Intermediate CD27 expression is seen compared with the isotype.

Additional Notes

None

Related Categories

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Summary

The Good

Use based on the company's test volume

The Bad

Could be brighter

The Bottom Line

Good antibody for detection of CD27 on dendritic cells.

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