Great, Reliable ELISA

University of California, San Diego
Department of Pediatrics
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Mouse CXCL1/KC DuoSet ELISA

Catalog Number:

DY453-05

We analyzed murine lung homogenate supernatant, detection of KC was easy in infected samples.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Lung homogenate

Protocol Overview

Mice were infected with M. tuberculosis for 3 weeks. Lungs were collected and homogenized in 5mL PBS-T. 1mL of homogenate was aliquoted and spun down at max speed, and the supernatant was filtered through a 0.2um PVDF filter. Sample was diluted 1:3 in the appropriate buffer as per product guidelines.

Tips

May need to try several dilutions to get your sample within the standard curve.

Results Summary

Results were consistent and product is very easy to use.

Additional Notes

None

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Summary

The Good

Works consistently

The Bad

None

The Bottom Line

I like their ELISAs, they have been very consistent and are really easy to use.

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