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APC/Cy7 anti-mouse F4/80
123117
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We are interested in studying macrophage activation phenotypes in mice. We collect peritoneal exudate cells, and use flow cytometry to gate out the non-macrophage population. This antibody provides a bright signal on a channel that was useful for our panel.
Flow Cytometry
Mouse peritoneal exudate cells
1:100 of each Fc block + FITC anti-mouse CD19 + Brilliant Violet 421™ anti-mouse CD11b + APC-Cy7 anti-mouse F4/80 on ice for 20 minutes.
1x PBS + 2% FBS + 2mM EDTA
NA
BD LSR Fortessa
Cells that are CD19 negative and CD11b positive are also F4/80 positive. This is a population of macrophages. Using the F4/80 stain, we can be more confident in the purity of our gating.
None
Clear, consistent results.
No drawbacks observed.
I would recommend adding this product to any laboratory working with mouse peritoneal macrophages.