Jackson ImmunoResearch Laboratories, Inc.
Rabbit anti dog IgG
304-095-003
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The aim of this experiment was to determine the proportion of RBC's bound to IgG from dogs that have Immune mediated hemolytic anemia. This antibody works incredibly well for doing this.
Flow Cytometry
Whole blood in EDTA
Add 100ul of 10g/ml conc. of antibody to 100 ul of washed RBC's.
NA
FACS CAlibur
The results generated by flow cytometry are in the form of percent positive or negative. In the case of dogs that are suspected of IMHA, we do see a positive population of cells bound to IgG. The percentage of these cells varies from dog to dog.
None
Works well for our assay
Good antibody.