CD80 Expression by Raji Cells

March 03, 2016

BBM III e Inmunologia
Universtity of Granada
Post-doc

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Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-human CD80 Antibody

Catalog Number:

305205

Our work involves the characterization of human fibroblast from different tissues and their relation with leukocytes. In this case, we analyzed the expression of CD80 by Raji cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Raji cells

Primary Incubation

1ug/ml in PBS suplemented with 1%BSA; 1 hour at RT

Blocking Agent

1% BSA + 4% human serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACScalibur

Results Summary

Gating on forward/side scatter alone gives a uniform population with respect to CD80. CD80 is shown in blue and isotype control is shown in red

Additional Notes

None

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Summary

The Good

No background

The Bad

None

The Bottom Line

It is an easy to use antibody that delivers good results

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